Analysis Note

Low-end CV% Average: 8%|Low-end CV% Range: 2 - 15%

Application

The sensitivity of Single Molecule Counting (SMC^®) gives the SMC^® Human Phospho-AKT1 Immunoassay Kit unparalleled performance in quantifying pAKT1 in cell lysates.

Research CategoryInflammation & Immunology

Components

PHOSPHO AKT1 Coated BeadsSample DiluentPHOSPHO AKT1 Detection AntibodyAssay BufferAKT1 StandardAKT1 Detection Antibody Buffer10X Wash BufferElution Buffer BBuffer DSMC^® PHOSPHO AKT1 Immunoasay Kit Instructions

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

General description

The serine-threonine protein kinase AKT family is the primary downstream mediator of the phosphatidylinositol-3-kinase (PI3K) signaling pathway. Members of the family (AKT1, AKT2, and AKT3) require two separate kinases for complete activation. AKT1 phosphorylates mTOR, whose downstream targets include other kinases, cell cycle regulators and transcription factors that are essential for many cell proliferation and survival processes. AKT1 also up-regulates matrix metalloproteinases and is thus implicated in tumor progression. AKT1 and other members of the AKT family have been an important focus in therapeutic development with their broad effects on the PI3K pathway.

The SMC^® pAKT1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC^® immunoassay system detects analytes using Single Molecule Counting (SMC^®) technology.

Legal Information

SMC is a registered trademark of Singulex